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I. A project on Blakeslea trispora,
for production of high quantity of beta carotene:
Carotenoids are a class of natural fat-soluble pigments
that is very widespread and which accurs in all the three
domains of life that is in the eubacteria, the archea and
in the eucarya. Carotenoids play major roles in human health
by acting as biological antioxidants protecting cells and
tissues from the damaging effects of free radicals. The
industrial production of natural carotenoids is through
the exploitation of some microorganisms which can synthesize
large amount of carotenoids. One such industrial source
of beta carotenoids is Blakeslea trispora.
Blakeslea trispora is a zygomyete mold. The organism is
a saprophyte that performs its negative cycle of spores,
filamentous mycelia, limiting bodies and again spores on
chemically defined media in the laboratory. Beta-carotene
is obtained from B .trispora by co- fermentation of 2 sexual
types of the fungus in specific propositions. These source
organisms are neither pathogenic nor toxinogenic. Both types
are stable in cultures maintained under conditions consistent
with good manufacturing practices. The compound is isolated
from the fungal biomass by solvent extraction and crystallized.
Technical
Programme
1) Screening of different media for high carotene production.
2) Use of different mutagens for high carotenoid production.
3) Increasing the amount of carotene content by using promoter/
elicitor
4) Characterization of the carotenoid produced by the above
methods.
2.
Effluent Treatment:
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Textiles: Increase recycled water utilization
(Currently around 45 to 50%) in garment processing units,
by studying the effluent treatment processing process.
By use of biochemical and probiotic means.
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Distilleries: Increase biomethanation
efficiency in distillery effluent processing (currently
65%) .Reduce color of final effluent caused by lignin
content.
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Paper: Reduce color, COD and BOD levels
of paper effluents
3.
Manufacturing of Enzymes, Herbal Extracts and Teaching Kits:
Enzyme: Enzymes have been used since dawn
of mankind in cheese manufacturing and indirectly via yeasts
and bacteria in food manufacturing. Many of the life processes
that biotechnology can make use of are chemical reactions
that are controlled by enzymes, which are proteins that
act as catalysts to speed up and direct the reactions. Some
biotechnological processes use whole organisms (e.g. yeasts
to make wine), while others use enzymes that have been purified
from the organism that makes them (e.g. enzymes in detergents
to dissolve grease and protein).
The enzyme production process can be divided into following
phases
1. Selection of enzyme
2. Selection of production strain
3. Construction of an overproducing strain by genetic engineering
4. Optimization of culture medium and production conditions
5. Optimization of recovery process (and purification if
needed)
6. Formulation of a stable enzyme product
Enzymes
1. Alfa-amylase 2. Protease
3. Peroxidase 4. Catalase
5. Bromelian 6. Papain
7. Lipases 8. Acid phosphates
9. Alkaline phosphotase
10. Cellulose 11. Urease
12. Trypsin 13. Lysozyme
Herbal
Extracts
Our herbal extraction processes work with the goal of producing
natural herbal extracts, which are a true representation
of the original plant. Studies confirm that botanical medicines
work better when the whole complex of plant phytochemicals
are kept together in extract form rather than by isolating
and concentrating a key component of the herb. From our
initial maceration of the botanical to the final product,
Wingene follows extraction techniques that preserve the
plant's phytochemical profile and effectiveness of the natural
medicine.
Wingene’s
herbal extraction techniques:
• Guarantee a standardized pharmaceutical grade product
in its' original natural chemical formation.
• Eliminate Bacteria and free radicals without residues,
chemicals, etc.
• Leave almost all the phyto chemicals and active
ingredients in the plant.
• Work with single or multiple botanical products.
• Create the most natural product you can achieve.
Some of the plants we use for herbal extraction are as follows:
• Tomato – Lycopersicum esculentum
• Carrot – Daucus carota
• Pepper – Pipera nigrum
• Turmeric – Curcuma longa
• Nutmeg – Myristica fragrans
Teaching
Kits:
Wingene is committed to advancing towards providing innovative
and quality products for biotech education. Addressing the
growing need for teaching kits in the student community
and Immuno-diagnostic Centers, Wingene has ushered in a
slew of Teaching Kits that make dealing in frontier areas
of Molecular Biology and Biotechnology a pleasure to learn
and explore.
1. Molecular Biology Teaching Kits
2. Protein Purification & Analysis Teaching Kits
3. Immunology Teaching Kits
4. Chromatography Teaching Kits
4.Quality
Control and Quality Analysis
An enzyme assay lab is established to do assay for the following
enzymes
1.
Alpha amylase 2. Acid and Alkaline Phosphatase
3. Bromelian 4. Catalse
5. Cellulose 6.Chymotrypsin
7. Chitinase 8. Beta galactosidase
9. Glucose Oxidase 10.Hemicellulase
11. Lipase 12. Lysozyme
13. Papain 14. Peroxidase 15.
Pectinase 16. Protease
17. Phytase 18. Superoxi Dismutase
19. Trypsin 20. Thrombin
21. Urease 22. Xylanase
Aside from these, we offer a whole lot more that aren’t
big enough to be categorized but important enough to find
a mention here:
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We create value for our students and customers, at every
step.
• We deliver products, services, or solutions which
drive customer-satisfaction and retention, and which catapult
our students’ career graph.
• We use customer-perceived value as the key criterion
for the design of current and future products and services.
• We challenge the status-quo to identify ways of
doing things better and faster.
• We eliminate boundaries to the sharing of ideas
and successes.
• We establish mutually beneficial objectives and
accountabilities to achieve goals.
• We function as both team player and leader.
• We provide a steady leadership.
• We assure clarity and agreement on performance expectations.
• We ensure our students learn from highly regarded
industry experts, network with their peers, and gain in-depth,
hands-on knowledge that you they can use immediately on
the job.
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